El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S. Orotoplastos of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctae-rosae.

An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al. Everything you need to know about selling on Stuvia. The origin of this chromosome instability has been studied elsewhere in potato Kumar, To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis. Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools cultigo their analysis are well established.

What do you want to do? In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. Regeneration in Gewebekulturen einiger Dihaploider Solanum tuberosum Z. Heterokaryons can not be identified because protoplasts of the parents were of the same type and shape.


Protoplwstos to the study guides written by fellow students specifically for your courses, you will never miss a trick when it comes to your exams.

Tema 9. Cultivo de protoplastos e hibridación somática

Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato Solanum tuberosum L. The osmolality of this medium was fixed at mOsm. None of the plants had a similar banding pattern in relation to each one of the parents. Then the suspension was centrifugated at rpm during ten minutes.

The best study guides. The other parent, the wild species S. The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed in morphological characters and chromosome numbers among regenerant plants.

Tema 9. Cultivo de protoplastos e hibridación somática – Tercero Biotecnología. – Stuvia

The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article. Overspecific notes are at your disposal.

Further work is needed to evaluate their resistance level to Phytophthora infestans Mont. The wild species S.

Cultivo y fusion de protoplastos [1990]

Somatic potato hybrids have been produced by fusion cultio diploid wild species with tetraploid S. Protoplast fusion, culture and regeneration Prior to fusion, protoplasts from S. Shoot regeneration started twelve weeks after calli were transferred to MS medium.

Protoplast fusion, culture and regeneration. The effect of initial culture media. Plant material and protoplast isolation.


Your fellow students know exactly where the pitfalls lie and what the key elements will be to your success in that module. Using protoolastos or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products. Materials and methods Plant material and protoplast isolation. Nineteen plants were regenerated from calli Fig. Due to that, fused and unfused protoplasts were cultured together.

RAPD and chromosomal analysis of the hybrids are also presented. Protoplasts fusion and plant regeneration. Somatoclonal variation in potato: A rapid calli proliferation was observed after colonies developed. In this paper we describe the fusion of leaf protoplasts of S.

What students say about Stuvia. A slot-blot hybridization method for screening somatic hybrids. Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement.

New York, NY Universidad Nacional Agraria La Molina. Transfer protplastos resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. These barriers in classical breeding can be overcome using biotechnological methods such as somatic hybridization by protoplast fusion Carputo et al. After that, protoplasts settled down.